STUDY GUIDE FOR PEST CONTROL ADVISERS: NEMATODES

IV. METHODS OF SOIL SAMPLING AND NEMATODE EXTRACTION

A. Sampling Considerations

1. Identify the appropriate time of sampling for:
• a. annual crops; (REVIEW)
• b. vineyards or orchards; (REVIEW)
• c. ornamental plantings or turf. (REVIEW)
2. List the background information that should be included when sending a sample to a lab. (REVIEW)
3. List the factors that determine the size of the sample to be taken. (REVIEW)
4. Define sample size and list the number of soil cores that make up a basic minimum sample. (REVIEW)
5. List the factors to consider when stratifying the field. (REVIEW)
6. List the minimum cores/5 acres and the core depths for samples taken in:
• a. field or row crops; (REVIEW)
• b. vineyards or orchards (preplant). (REVIEW)
7. Describe how to take a sample in:
• a. nursery stock; (REVIEW)
• b. a field. (REVIEW)
8. Identify the tools necessary to take a:
• a. soil sample; (REVIEW)
• b. root sample. (REVIEW)
9. Describe how to package a soil sample. (REVIEW)

1. Describe how to collect field samples for:
• a. established crops; (REVIEW)
• b. trees; (REVIEW)
• c. vineyards; (REVIEW)
• d. fallow fields. (REVIEW)
2. Describe storage and shipping requirements necessary to send a soil sample to a lab. (REVIEW)

1. List the major limitations of using soil sample results for practical nematode predictions. (REVIEW)

1. Recognize the importance of telling the lab the previous, current and subsequent crop to help them determine the extraction method. (REVIEW)
2. Recognize the importance of knowing the extraction procedure to interpret laboratory results. (REVIEW)
3. Describe why ectoparasitic nematodes are not found in root samples. (REVIEW)
4. Describe why root-knot nematodes may not be found in a root sample that has nematode galls on it. (REVIEW)

1. List the common units used to report nematode population levels from soil and from roots. (REVIEW)

1. Describe the factors that influence the efficiency of the extraction method. (REVIEW)
2. Explain extraction efficiency and its importance in nematode sampling. (REVIEW)

1. Identify the different extraction methods used by labs. (REVIEW)
2. List the preferred extraction method for:
• a. nematodes of foliage or stems; (REVIEW)
• b. dagger nematodes; (REVIEW)
• c. cyst nematodes. (REVIEW)
3. List the factors to consider in conjunction with every nematode sample. (REVIEW)
4. Describe how to estimate the expected crop yield loss from the labreport. (pg. 103-109 IPM for cotton) (REVIEW)

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