TIME OF THE YEAR TO SAMPLE:

The best time of the year to sample varies for each cropping situation.

ANNUAL CROPS

In annual cropping situations, nematodes typically are at a low level during planting and increase towards harvest as long as roots are healthy enough to support nematode feeding. This example of root-knot nematode Meloidogyne chitwoodi on potatoes in Tulelake, CA in which populations of juveniles were monitored at weekly intervals from planting in May until harvest in October illustrates this principle. Note that nematode numbers are plotted on a log scale.

Following harvest, nematode numbers typically decrease until another susceptible crop is planted or weeds become available that will support reproduction. Under weed free fallow conditions, root-knot nematode populations may drop as much as 85-90 percent in a years time, while sugarbeet cyst nematode will only decrease by 20 to 60 percent depending on location. This principle is illustrated in this example of population decrease of sugarbeet cyst nematode following harvest of a susceptible crop until the planting of another susceptible crop (cabbage) during which time populations increase dramatically and then decrease once again during successive nonhost crops.

Nematode populations are usually easier to detect at harvest when they are highest than several months following harvest. For example, after several months in a fallow or nonhost crop, populations of root-knot nematode may not be detectable through sampling but may still be high enough to cause significant damage when a host is planted.

PERENNIAL CROPS

Perennial crops will typically fluctuate during the year with the timing of the fluctuations varying by crop and location. Only a few perennial crops in California have been sampled over prolonged periods. These include grapes (McKenry and Ferris in Fresno County, Radewald in Riverside County, and Feil and Westerdahl in San Joaquin and Sonoma Counties), peaches ( Lownsbery and Ferris in separate trials in Merced County), almonds ( Asai and Westerdahl in Stanislaus County), and prunes (Westerdahl and Farm Advisors in Glenn, Sutter, Tehama, Shasta, and Colusa Counties).

Because populations fluctuate, if one wants to compare populations from year to year, it is important to always sample at the same time each year. Also, if treatments are applied to try to reduce nematode numbers, its important to leave some areas untreated for comparative sampling. If this is not done, and samples are taken several months after a treatment, populations may be either lower or higher than before treatment just because of normal populations fluctuations.

ORNAMENTAL PLANTINGS OR TURF

Population fluctuations on turf will likely be similar to other perennial crops. Those on ornamental plantings will likely vary depending on whether the planting is an annual or a perennial.

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